FAQS

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Wondering how easy it is to add real-time optical sensors to your cell culture tissue flask or bioreactor? Gather pH and dissolved oxygen sensor insights from pericellular data.

  • Do the sensors come sterile?Yes. We provide both sterile and non-sterile sensors.
  • How can I sterilize the non-sterile sensors?Non-sterile sensors can be sterilized by autoclave, ethylene oxide, and gamma irradiation. Recalibration may be required after sterilization.
  • Are the sensors reusable?No. They are single use, disposable products.
  • I am going through a lot of sensors. Is there a high quantity price break?Yes. We offer price breaks for different tiers of high volume ordering. Please contact us for details.
  • Do I have to use one of each pH and DO sensors or can I use two pH or two DO sensors?You may use any combination of sensors that best suit your application. Make sure you indicate in the ID • Data Hub software the sensor type for each station.
  • Is there a benefit in using larger sensors?Generally, no. It is critical that the sensor is large enough to contain the entire ID • Reader LED beam. The minimum recommended sensor size is 4mm, although 6mm would be preferred.
  • I have a large culture vessel. Should I use larger sensors?No. You do not need larger sensors for larger vessels. However, you may consider using multiple sensors.
  • What’s the smallest sensor I can use?You can use a sensor as small as 4mm in diameter.
  • Does sensor shape matter?Sensor shape does not matter as long as the entire ID • Reader LED beam is contained within the sensor. Rectangular sensors may be easier to attach and detach from culture vessel surfaces.
  • How far can the sensor be from the ID • Reader?The smaller the sensor, the closer it should be positioned to the ID • Reader. For the smallest sensor with 4mm diameter, it should be within 1 cm from the ID • Reader. The distance between sensors and ID • Readers should be minimized.
  • What vessel materials do the ID • Readers work with?Clear glass such as borosilicate glass and most polycarbonate grades work well. Please contact us about your material.
  • What do I need to provide to use the ID •  Developer’s Kit?You will need to provide a computer with internet connection and the culture vessel with your cells and media.
  • What types of culture vessels does the ID • Developer’s Kit work with?The ID • Developer’s Kit works with a wide range of culture vessels. The vessel will need to have a flat surface for the sensor to attach on and the ability to position the ID • Reader across the sensor on the outside of the vessel. The distance between the two should be minimized and typically be around 1cm. The vessel material should also have suitable optical properties to allow for signal reading.

    We also offer fiber optic ID • Readers that work well with challenging culture vessel geometries. Please contact us to discuss your setup.
  • What expansion paths are possible? Can I use more sensors?You can have two sensors per ID • Reader and four ID • Readers per ID • Converter.
  • Can I use my own shake flasks?We provide shake flasks instrumented with the sensors but you can use your own shake flasks and purchase separately ID • Sensors suited for your flasks.
  • I have a different shaker I’d like to use. How can I add the sensors?You could add the sensors by purchasing an ID • Developer’s Kit. However, you will need to develop a method of securing the ID • Readers and flasks to your platform.
  • Can I place the ID • Shaker inside an incubator?The ID • Shaker includes temperature control. However, if desired, you can place it inside a non-condensing incubator within the specified temperature range.
  • How is the ID·Fiber Optic Kit different from the ID·Developer’s Kit?The ID·Fiber Optic Kit contains SBI’s adapted ID·Fiber Optic Reader with ID·Fiber Optic Cables. While the ID·Developer’s Kit is excellent for monitoring cell culture conditions in culture vessels that can be placed on top of an ID·Reader, the ID·Fiber Optic Kit and its accessories offer versatility in sensor placement and measurement readings in bioreactors, organ-on-a-chip devices, perfusion flow loops and other challenging geometries.
  • How many vessels can I monitor with an ID·Fiber Optic Kit?Each kit includes an ID∙Converter that supports up to 4 readers. With two sensing channels per reader, you can monitor up to 8 channels (pH and/or DO).
  • Does the ID·Fiber Optic Kit come with sensors?The ID·Fiber Optic Kit includes the ID·Fiber Optic Reader, ID·Fiber Optic Cables, ID∙Converter, and ID∙Data Hub software. Pair the kit with one of SBI’s Fiber Optic Accessories to integrate optical sensing into your bioprocess.
  • What accessory should I purchase for my application?Watch our ID·Fiber Optic Kit Accessories Overview video for more information.
  • What size port does the ID·Fiber Optic Probe fit?The ID·Fiber Optic Probe is the same size as a standard bioreactor port and fits a PG13.5 thread connection.
  • Do I have to calibrate the probe prior to use?Nope! Because the probe is designed to house SBI’s fiber optic cables and comes preloaded with ID·Sensors, you just insert the probe into your bioreactor and you’re ready to go.
  • Where are the sensors? Do I need to place them myself?The ID·Fiber Optic Probe comes with ID·Sensors for both pH and DO already attached to the end of the probe that will interact with the culture media. Each sensor is aligned to one of the three available channels, leaving an extra channel for temperature measurements.
  • Can I reuse the probe and sensors?The ID·Fiber Optic Probe and ID·Sensors are designed to be single use.
  • Where are the sensors? Do I need to place them myself?ID·External Star Adaptors come with a pack of your choice of ID∙Sensor pH or DO in a separate package. The ID·External Star Adaptor is placed on the outside of the culture vessels with the ID·Sensors placed on the inside wall.
  • How do I place the sensors?Use sterile forceps or one of SBI’s sensor placement tools to attach each ID·Sensor to the interior wall of your vessel.
  • How do I place the ID·External Star Adaptor so that it is centered over the sensor?Center the adherent sensor guide over the previously placed ID·Sensor and stick to the exterior of your vessel. Remove the adhesive from the ID∙External Star Adaptor, align to the sensor guide, and attach to the exterior of the vessel.
  • Can I reuse the ID·External Star Adaptor?The ID∙External Star Adaptor is a single use, disposable product. However, because it adheres to the exterior of your culture vessel and does not interact with the culture environment, it is possible to reuse it, but we cannot guarantee the integrity of the adhesive for multiple uses.
  • Can I reuse the ID·Sensors?ID·Sensors are designed to be single use and disposable.
  • What size tubing does the ID∙Flow Through Cell fit?The Luer fittings on the ID∙Flow Through Cell have a 3 mm flow ID. 3 mm diameter tubing is recommended for optimal readings.
  • Where are the sensors? Do I need to place them myself?The ID∙Flow Through Cells have the ID·Sensors already attached within the pipe portion of the units. The sensors are aligned to the channel that will secure the fiber optic cable in place.
  • Can I reuse the ID·Flow Through Cells?The ID∙Flow Through Cells and ID·Sensors are designed to be single use.

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