Microbial
Fermentation

Microbial Fermentation

Microbial bioprocesses play a key role in a broad variety of research areas such as synthetic biology and biopharma.

One of the key process parameters for any microbial bioprocess is biomass. In the past, biomass data was generated manually by investing hands-on-time to generate offline OD measurements.

With Our Cell Growth Quantifier (CGQ) and CGQ BioR technology, we have revolutionized biomass monitoring. Our sensor-based technology offers highly parallelized, non-invasive online monitoring of biomass in shake flasks, bioreactors and other cultivation vessels such as serum bottles. Simply attach our sensors to your cultivation vessel and generate detailed growth profiles of your organisms in real time.

Various leading research organizations and companies have used the CGQ and CGQ BioR for research and publications and are happy users of our technologies. For a more detailed overview, check out our Publications and Customer Success Stories!

But we do not stop at delivering insights. We want you to be able to control your bioprocesses. This is why we developed the Liquid Injection System (LIS), the first easy-to-use technology for automated feeding of liquids in shake flasks. Fill the single use sterile cartridge with up to 25mL of any type of liquid, define your feeding profile and install the cartridge and drive on the flask. LIS will now run your chosen feeding profile and help you unlock completely new experimental possibilities in shake flasks.

Yeast

Komagataella phaffii (Pichia pastoris)

From the very beginnings of discovery, all the way through industrial-scale production and commercialization, Pichia pastoris (since 2009 officially classified as Komagataella phaffi) has recently become well established for its rapid and cost-effective expression of recombinant proteins. Pichia has been used to successfully produce a wide variety of proteins including enzymes, antigens, recombinant antibody fragments (both fc and fab), and has proven beneficial as a tool for use as a next-gen protein scaffold.
The widespread adoption and application of Pichia for a variety of biological fields has made monitoring and controlling early and mid-stage cell culture parameters increasingly important.
The high oxygen demands of Pichia mean that cells can quickly go anoxic when not closely monitored. SBI’s dissolved oxygen optical sensors can be easily placed inside shake flasks with either the ID·Shaker or the ID·Shaker Deck Assembly and are ideal for early-stage propagation and optimization of cell culture parameters for selection and production scale-up.
The widespread adoption and application of Pichia for a variety of biological fields has made monitoring and controlling early and mid-stage cell culture parameters increasingly important. The high oxygen demands of Pichia mean that cells can quickly go anoxic when not closely monitored. SBI’s dissolved oxygen optical sensors can be easily placed inside shake flasks with either the ID·Shaker or the ID·Shaker Deck Assembly and are ideal for early-stage propagation and optimization of cell culture parameters for selection and production scale-up.

Saccharomyces Cerevisiae

Used in industries ranging from food sciences to drug delivery (and everywhere in between), Saccharomyces cerevisiae is one of the most adaptable and widely used yeasts around the world.
When environmental conditions are optimal, the budding yeast reproduces by mitosis as diploid cells. When cells are starved, they undergo meiosis and haploid spores are formed. The change from diploid to haploid, its ability to grow aerobically and anaerobically, and its ability to utilize different sources of sugar are highly dependent on controlling cell culture conditions.
From the early stages of upstream selection and transformation, through to fermentation, consistent environmental conditions are vitally important for desired cellular activity. SBI’s ID·Sensor pH and DO and ID·Shaker are great options for propagation, scale-up, industrial production, and optimization of cell culture parameters.
When environmental conditions are optimal, the budding yeast reproduces by mitosis as diploid cells. When cells are starved, they undergo meiosis and haploid spores are formed. The change from diploid to haploid, its ability to grow aerobically and anaerobically, and its ability to utilize different sources of sugar are highly dependent on controlling cell culture conditions. From the early stages of upstream selection and transformation, through to fermentation, consistent environmental conditions are vitally important for desired cellular activity. SBI’s ID·Sensor pH and DO and ID·Shaker are great options for propagation, scale-up, industrial production, and optimization of cell culture parameters.

Yarrowia lipolytica

Yarrowia lipolytica is an oleaginous yeast and has proved itself as an effective platform for the production of high quality and high value oleochemical products such as waxy esters and emollient oils. Capable of biosynthesizing long-chain fatty acids and fatty alcohols, Y. lipolytica is a useful host for heterologous protein production.
Considered a non-conventional yeast for protein production, Yarrowia is strictly aerobic and requires high concentrations of dissolved oxygen when grown at high cell density. Despite its constant oxygen demand, Yarrowia’s use continues to increase for research as well as industrial production because of its secretory activity and ability to produce important metabolites.
Despite Y. lipolytica’s ability to adapt to a wide range of pH conditions without impairing its growth or substrate uptake rate, pH should be tightly controlled as shifting of metabolite patterns has been observed in variable pH conditions. SBI’s ID·Sensors and ID·Shaker are ideal for optimization of cell culture parameters for proper protein secretion commercial production scale-up.
Considered a non-conventional yeast for protein production, Yarrowia is strictly aerobic and requires high concentrations of dissolved oxygen when grown at high cell density. Despite its constant oxygen demand, Yarrowia’s use continues to increase for research as well as industrial production because of its secretory activity and ability to produce important metabolites. Despite Y. lipolytica’s ability to adapt to a wide range of pH conditions without impairing its growth or substrate uptake rate, pH should be tightly controlled as shifting of metabolite patterns has been observed in variable pH conditions. SBI’s ID·Sensors and ID·Shaker are ideal for optimization of cell culture parameters for proper protein secretion commercial production scale-up.

Bacteria

Escherichia coli

E. coli is a Gram-negative, rod-shaped bacterium that uses oxygen when present and available but can also grow in the absence of oxygen using fermentation or anaerobic respiration, classifying it as a facultative anaerobe. Commonly found in the lower intestine, most E. coli strains are harmless. In laboratory settings, E. coli can be easily and inexpensively cultured and has been used as a research tool for more than 70 years.
As the most widely studied prokaryotic model organism and an important species in the fields of biotechnology, microbiology, and synthetic biology, E. coli has served as the host organism of choice for the majority of recombinant DNA work thanks to its rapid reproduction time.
With an optimal pH of 6.5-7.5 and the ability to grow in the presence or absence of oxygen, monitoring cell culture conditions are extremely important to ensure desired cellular activity. Real-time pH and dissolved oxygen sensing in shake flasks and other scale down or scale up vessels is made possible with ID·Sensors and the ID·Shaker for propagation, scale-up, industrial production, and optimization of cell culture parameters.
As the most widely studied prokaryotic model organism and an important species in the fields of biotechnology, microbiology, and synthetic biology, E. coli has served as the host organism of choice for the majority of recombinant DNA work thanks to its rapid reproduction time. With an optimal pH of 6.5-7.5 and the ability to grow in the presence or absence of oxygen, monitoring cell culture conditions are extremely important to ensure desired cellular activity. Real-time pH and dissolved oxygen sensing in shake flasks and other scale down or scale up vessels is made possible with ID·Sensors and the ID·Shaker for propagation, scale-up, industrial production, and optimization of cell culture parameters.

Bacillus subtilis

Bacillus subtilis, also known as grass or hay bacillus, is one of the most well studied Gram-positive bacterium and has served as model organism for research in biochemistry, molecular biology, cell differentiation, genetics, and chromosomal replication.
Its unique secretory pathway has helped establish B. subtilis as an important platform for production of enzymes and fine chemicals such as vitamin B12 and nucleotides. Thanks to the relative ease of genetic manipulation and favorable growth characteristics, the rod-shaped bacterium is an attractive organism for industrial applications and fermentation sciences.
With a typical working pH of 7.5, SBI’s ID·Sensors for pH and DO, ID·Shaker, and ID·Shaker Deck Assembly are perfect options for genetic manipulation, propagation, scale-up, and optimization of environmental conditions for this versatile cell factory.
Its unique secretory pathway has helped establish B. subtilis as an important platform for production of enzymes and fine chemicals such as vitamin B12 and nucleotides. Thanks to the relative ease of genetic manipulation and favorable growth characteristics, the rod-shaped bacterium is an attractive organism for industrial applications and fermentation sciences. With a typical working pH of 7.5, SBI’s ID·Sensors for pH and DO, ID·Shaker, and ID·Shaker Deck Assembly are perfect options for genetic manipulation, propagation, scale-up, and optimization of environmental conditions for this versatile cell factory.

Everything We Do is Bio

Advances in synthetic biology are using plasmid transformation and protein secretion in ways not possible just a few short years ago, and the field is becoming a more important part of the bioeconomy. With bioengineering and bioprocessing playing an increasingly important role in the fields of food and feed, biofuels, medicine, bioplastics, renewable energy, and more, now is the time to make plans that will lead to success at every step along the way.
Contact us today to talk about your microbial culture application and how real-time sensing of pH and dissolved oxygen can improve your experimental outcomes.

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