Introducing the DOTS Platform for Cell Culture! Learn more here.

Overview

As the central piece of sbi’s DOTS Platform, the MPS enables effortless online monitoring of multiple parameters in shake flasks. From dissolved oxygen (DO) and biomass monitoring to fluorescence detection – the MPS turns the standard shake flask into smart bioreactors.

Key Features

  • Monitor a broad range of parameters: Biomass, Fluorescence, Dissolved Oxygen (DO), and More!
  • Easy to install under each shake flask
  • Combine with our LIS Flask Feeding technology for smart feeding applications like DO-based feeding

Benefits

  • Real-time, actionable insights from all your shake flasks
  • Save time with automated, online monitoring of many shake flasks in parallel
  • Say goodbye to manual sampling
  • Run bioreactor-like experiments on shake flask level
  • Use with any shake flask for reduced operating costs

Compatible With Your...

Bioprocess
  • For a broad range of organisms and CHO cell types
  • For most media compositions
Lab Infrastructure
  • For different shake flask sizes
    • From 100 mL - 2000 mL
  • For different shake flask types
    • Nalgene, Corning, Glass
  • Compatible with every shaking incubator
    • Clamps and Sticky Mats

  • For a broad range of shaking speeds and filling volumes

Applications

  • Bioprocess characterization

  • Cell Line/Strain Development, Characterization, and Selection

  • Collect critical process parameters for an informed upscaling process

  • Optimize process conditions with parameter-based feeding

How It Works

Our Multiparameter Sensor (MPS) enables dissolved oxygen, backscatter (biomass), and fluorescence monitoring in shake flasks. The platform-type sensor is built to accommodate additional sensor parameters as they are developed by the sbi team, ensuring scientists will have the latest technology.multiparameter-sensor-for-shake-flasks-sbi

 

Application Data & Results

Explore applications in real-world situations. Looking for something specific? Contact us to see data for your organism and application.

Microbial

Metabolic Event Detection
Data Spotlight - Monitoring Metabolic Events Through the Detection of eGFP Expressed by E. coli (1)

Escherichia coli (E. coli), 200 rpm/25 mm, 10 % filling volume, 250 ml flask, 37°C

DOTS enables real-time monitoring of multiple parameters. Early IPTG inoculation (marked by the red triangle) is used to induce protein expression, showing that GFP expression is growth-coupled. By continuously monitoring these parameters online, researchers can gain insights into the dynamics of metabolic events occurring within the shake flask culture.

Cell Culture

 
CHO_cell_biomass_Total_cell_density

CHO Cell, 0.3 million cells / mL, Nalgene flask, 250 mL, 20% filling volume (50 mL), 150 rpm, 50 mm

Biomass for CHO cells demonstrates strong correlation with offline biomass measurements obtained via Total Cell Density (TCD) analysis. The MPS exhibits exceptional reproducibility when comparing data collected from two independent sensors (Flask 1 vs Flask 2), showcasing its consistency and robustness for process monitoring in bioprocess applications.

Want to see data for your organism/cell line or application?

We have tested over 100 organisms/cell lines for more than 50 different applications with our technology.

Speak With An Expert

Hardware & Software Components

Multiparameter Sensor & Adapter

multiparameter-sensor-adapter-icon (2)
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Each sensor plate is positioned in the adapter under the cultivation vessel and measures non-invasively through the vessel wall.

USB Hub

usb hub in grey circle
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Several sensor plates can be connected to a single hub. The hub bundles the data from all monitored flasks and sends it to the DOTS Software.

DOTS Software

SBI-Icon-dots-software-computer-2
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The DOTS Software receives the data from the hub and visualizes relevant parameter data in real-time.

What Our Customers Are Saying

"You don't get more product by trying to get more product, but by gaining more insights into your process."

-Raul Reveles (Senior Bioprocessing Engineer, Bond Pet Foods)
Bond Pet Foods Logo white

From Estimation To High-Resolution Growth Curves

Why CGQ for your Shake Flasks?

Manual sampling-based data is often not sufficient to fully understand the bioprocess. Offline sampling is complex and time consuming, resulting in lower measurement frequency with most pulls being at the start or the end of the experiment. This means that critical information from your growth phases are being overlooked, and could have a detrimental impact on your final product. Automated online measurements, on the other hand, never miss a moment. With a high resolution growth curve, you can detect bioprocess changes in real-time.

Cell Growth Quantifier (CGQ)
Success Growth Graph

Customer Success Stories

SBI-Success-Table-2
"Our group has been using the Cell Growth Quantifier (CGQ) for roughly five years by now and even though we often use the device for screenings, it also is the perfect tool for toxicity tests.”

-Dr. Julia Hitschler (University of Frankfurt)
Goethe University Frankfurt
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Cell Growth Quantifier (CGQ)
"The backscatter data we gathered here with the CGQ system clearly shows a match with another growth characterisation parameter, in this case with the oxygen transfer rate (OTR)."

-Frédéric Lapierre (HS München)
HM
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Want To Connect The DOTS In Your Bioprocessing?

CONTACT OUR TEAM