"Pericellular oxygen concentration is in a range that most would consider severe hypoxia, at <0.6% or about 4.6mm Hg, in the medium in 21% oxygen atmosphere."
Placenta, Vol 24(2), 2013. pp. 106-109.
70% of scientists surveyed recently by Nature were unable to reproduce another’s experiment, and a post-publication analysis has suggested a reproducibility rate of as little as 10% in cancer biology.
Commun Biol, Vol 2, 2019. 144.
Dissolved oxygen concentration at the bottom of the flask reached zero during cell growth which indicates that mixing and mass transfer is not optimal for T-flask cultured cells. Despite these significant achievements, on-line monitoring of both DO and pH changes during cell passaging or subculturing in T-flasks and how they may affect cellular metabolism is not routine.
Biotechnology and Bioengineering. 105(6): 1040-72.